Abstract：Work-life balance has become a widely discussed global issue and a focal point of scholarly attention. However, existing studies on work-life balance in educational settings have primarily focused on general teachers, while research specifically examining physical education (PE) teachers in schools has been relatively scarce. This study aimed to explore the relationships among work-home culture, work-to-family facilitation, work-life balance, and person-organization fit from the perspective of work-family facilitation. Purposive sampling was adopted to recruit PE teachers in secondary and primary schools in Taiwan. The results indicated that the perceived work-home culture of PE teachers had a positive correlation with work-life balance. Meanwhile, work-home culture was significantly related to work-life balance through the mediating effect of work-to-family facilitation. Finally, a high person-organization fit could strengthen the mediating relationships among work-home culture, work-family facilitation, and work-life balance. However, low person-organization fit leads to insignificant mediating effects instead. This current study explores the relationships between cross-field interactions between work and family. Also, it provides a positive theoretical perspective for research on work-family interfaces in education. In practical suggestions, aside from creating a family-friendly workplace atmosphere, the study encourages PE teachers to integrate family activities into their work. When PE teachers strongly align with school policies and fit well within the organization, the work-home culture created by the school can significantly enhance the positive impact of work on families, leading to a win-win balance between work and life. (Full text)